ISMac25
- Family IS1
- Group ISMhu11
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_003552 | ND | Methanosarcina acetivorans | Methanosarcina acetivorans C2A |
DNA section
IS Length : 1157 bp
Ends
IR Length : 21/30
IRL : GGTCAATTTGTATTTGATTGGTATTATATATTAGAAATTATATGTTCATA
IRR : GGTCCATCTTTATCCGATTGATGTTTTAAAACACCTGAATTTCATCAGCT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TTGCATTCAACAAGAATGAT | ATCACAAGAATGCTAATGAAATA | 0 | |
AACTGAGTAATTCTTTTGAT | ATCGAAGGTTTGAGGTGATT | 0 | |
CATGATATGGAAGGTTTGAT | ATCGAAGGTTTTGAATCACC | 0 |
DNA sequence
GGTCAATTTGTATTTGATTGGTATTATATATTAGAAATTATATGTTCATATTATGGGAAAAAGAGGTCCTAAACCACAATTTACTGACGTTGCCTGTCCG
AACAAAAGCTGCGAGCTCTACGGCCTTACTGGTCAAGGCAATGTTACTGGAAATGGAACTTACATCAGCCGTGGAGAAAAAACAAGAATATATCGTTGCA
ATGAATGTGGCAAAGCATTTTGCGACCATACAGGCACTTTTTATCATGATCTTCGCAAAGATGATAAAACAATCGATTTAGCTTTAAAAATGTCTATGAA
AGGTATGAGCATTCAAGCCATTGCCGAAGTTTTAGAGGTACAACCTGCCAGTGTAAAGCGCTGGCTAGCCCGTGCAGCTGAACAATGCGATAAACTAAAT
GATACTCTGATGAAGAACGTGGATGTGTTAAAGGTAGAAATGGACGAACTATGGGTTATAATCCAAAAAAATAGTTCCAAGAATGAAAAATTATGAAGAT
GATGGGCCCTGGATGTGGGTAGCTTTTGTACCAGGTTGCAGGCTAATACTTGACTTTGTAATAGGGCCAAGAAAGCAGTATGTTGCGGATAAGTTTATTG
AGTTAGTTAATAAGCATATTTCGGATAAAATCCCTGTTTTTGTCACAGATGGGTTGAACTTTTATAGAGAAGCGCTTTTGAAACAATTTGGAGTTTTGAG
AGAGTTTCCAAGAACTGGGAAAAGAGGAAGACCAAAGAAACCAAAAATAGTTCCCTCTGAGGATTTGAGGTATGCTCAGGTAGTCAAAACAAGAGTAAAT
GGGGTGCTTGAGAAAGTAGAGAAGAAGATCATTTTTGGAGAAAACATTGAACAAAGCGAAATCTCAACAACTTTACTGGAAAGACAAAACCTAACCTTCA
GGCAGGATAACAACAGAGTGTCAAGGAAAACAATAGGGTTTTCAAAAATGAAAGAATGGTTAGAAATACAGATGAAGCTCTATTGCACGCATTTTAACTT
CTGTAGAGGGCATGGAGGATTAAGGTACAAAGATGAAAGAGGGGTTGAATGCAAAAATACACCTGCAAGAAAAGCAGGAATTGCGGATTCAAAATGGACT
TTGAAGGAGCTGATGAAATTCAGGTGTTTTAAAACATCAATCGGATAAAGATGGACC
AACAAAAGCTGCGAGCTCTACGGCCTTACTGGTCAAGGCAATGTTACTGGAAATGGAACTTACATCAGCCGTGGAGAAAAAACAAGAATATATCGTTGCA
ATGAATGTGGCAAAGCATTTTGCGACCATACAGGCACTTTTTATCATGATCTTCGCAAAGATGATAAAACAATCGATTTAGCTTTAAAAATGTCTATGAA
AGGTATGAGCATTCAAGCCATTGCCGAAGTTTTAGAGGTACAACCTGCCAGTGTAAAGCGCTGGCTAGCCCGTGCAGCTGAACAATGCGATAAACTAAAT
GATACTCTGATGAAGAACGTGGATGTGTTAAAGGTAGAAATGGACGAACTATGGGTTATAATCCAAAAAAATAGTTCCAAGAATGAAAAATTATGAAGAT
GATGGGCCCTGGATGTGGGTAGCTTTTGTACCAGGTTGCAGGCTAATACTTGACTTTGTAATAGGGCCAAGAAAGCAGTATGTTGCGGATAAGTTTATTG
AGTTAGTTAATAAGCATATTTCGGATAAAATCCCTGTTTTTGTCACAGATGGGTTGAACTTTTATAGAGAAGCGCTTTTGAAACAATTTGGAGTTTTGAG
AGAGTTTCCAAGAACTGGGAAAAGAGGAAGACCAAAGAAACCAAAAATAGTTCCCTCTGAGGATTTGAGGTATGCTCAGGTAGTCAAAACAAGAGTAAAT
GGGGTGCTTGAGAAAGTAGAGAAGAAGATCATTTTTGGAGAAAACATTGAACAAAGCGAAATCTCAACAACTTTACTGGAAAGACAAAACCTAACCTTCA
GGCAGGATAACAACAGAGTGTCAAGGAAAACAATAGGGTTTTCAAAAATGAAAGAATGGTTAGAAATACAGATGAAGCTCTATTGCACGCATTTTAACTT
CTGTAGAGGGCATGGAGGATTAAGGTACAAAGATGAAAGAGGGGTTGAATGCAAAAATACACCTGCAAGAAAAGCAGGAATTGCGGATTCAAAATGGACT
TTGAAGGAGCTGATGAAATTCAGGTGTTTTAAAACATCAATCGGATAAAGATGGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
444 bp | 147 aa | 53 | 496 | + | No |
Description : First part of the transposase
ORF sequence :
MGKRGPKPQFTDVACPNKSCELYGLTGQGNVTGNGTYISRGEKTRIYRCNECGKAFCDHTGTFYHDLRKDDKTIDLALKMSMKGMSIQAIAEVLEVQPAS
VKRWLARAAEQCDKLNDTLMKNVDVLKVEMDELWVIIQKNSSKNEKL
VKRWLARAAEQCDKLNDTLMKNVDVLKVEMDELWVIIQKNSSKNEKL
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
687 bp | 228 aa | 462 | 1148 | + | No |
Description : Second part of the transposase
ORF sequence :
SKKIVPRMKNYEDDGPWMWVAFVPGCRLILDFVIGPRKQYVADKFIELVNKHISDKIPVFVTDGLNFYREALLKQFGVLREFPRTGKRGRPKKPKIVPSE
DLRYAQVVKTRVNGVLEKVEKKIIFGENIEQSEISTTLLERQNLTFRQDNNRVSRKTIGFSKMKEWLEIQMKLYCTHFNFCRGHGGLRYKDERGVECKNT
PARKAGIADSKWTLKELMKFRCFKTSIG
DLRYAQVVKTRVNGVLEKVEKKIIFGENIEQSEISTTLLERQNLTFRQDNNRVSRKTIGFSKMKEWLEIQMKLYCTHFNFCRGHGGLRYKDERGVECKNT
PARKAGIADSKWTLKELMKFRCFKTSIG
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1096 bp | 365 aa | 53 | 1148 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKRGPKPQFTDVACPNKSCELYGLTGQGNVTGNGTYISRGEKTRIYRCNECGKAFCDHTGTFYHDLRKDDKTIDLALKMSMKGMSIQAIAEVLEVQPAS
VKRWLARAAEQCDKLNDTLMKNVDVLKVEMDELWVIIQKKIVPRMKNYEDDGPWMWVAFVPGCRLILDFVIGPRKQYVADKFIELVNKHISDKIPVFVTD
GLNFYREALLKQFGVLREFPRTGKRGRPKKPKIVPSEDLRYAQVVKTRVNGVLEKVEKKIIFGENIEQSEISTTLLERQNLTFRQDNNRVSRKTIGFSKM
KEWLEIQMKLYCTHFNFCRGHGGLRYKDERGVECKNTPARKAGIADSKWTLKELMKFRCFKTSIG
VKRWLARAAEQCDKLNDTLMKNVDVLKVEMDELWVIIQKKIVPRMKNYEDDGPWMWVAFVPGCRLILDFVIGPRKQYVADKFIELVNKHISDKIPVFVTD
GLNFYREALLKQFGVLREFPRTGKRGRPKKPKIVPSEDLRYAQVVKTRVNGVLEKVEKKIIFGENIEQSEISTTLLERQNLTFRQDNNRVSRKTIGFSKM
KEWLEIQMKLYCTHFNFCRGHGGLRYKDERGVECKNTPARKAGIADSKWTLKELMKFRCFKTSIG
Blast result :
Comments
ISMac25 is 37% aa similar to ISDge2. The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Galagan,J.E., Nusbaum,C., Roy,A., Endrizzi,M.G., Macdonald,P., FitzHugh,W., Calvo,S., Engels,R., Smirnov,S., Atnoor,D., Brown,A., Allen,N., Naylor,J., Stange-Thomann,N., DeArellano,K., Johnson,R., Linton,L., McEwan,P., McKernan,K., Talamas,J., Tirrell,A., Ye,W., Zimmer,A., Barber,R.D., Cann,I., Graham,D.E., Grahame,D.A., Guss,A.M., Hedderich,R., Ingram-Smith,C., Kuettner,H.C., Krzycki,J.A., Leigh,J.A., Li,W., Liu,J., Mukhopadhyay,B., Reeve,J.N., Smith,K., Springer,T.A., Umayam,L.A., White,O., White,R.H., Conway de Macario,E., Ferry,J.G., Jarrell,K.F., Jing,H., Macario,A.J., Paulsen,I., Pritchett,M., Sowers,K.R., Swanson,R.V., Zinder,S.H., Lander,E., Metcalf,W.W. and Birren,B. (2002) Genome Res. 12 (4), 532-542.