ISfinder

ISOba3

Family IS1
Group ISMhu11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAMO01000002	ND	Oceanicola batsensis	Oceanicola batsensis HTCC2597

DNA section

IS Length : 2598 bp

Ends

IR Length : 26/28

IRL : GGTAGTGGGTCAGTTTGAAATCTAGCCCCTCGCGCAAACGCGCAGCTTTG
IRR : GGTAGTGGGTCAGTTTGAAATCTTGGCCTGTTACAACCCTTGCAACGCAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGTCACAGGC	CCCGCCTG	AGAATGCTGGACCCG	8

DNA sequence

GGTAGTGGGTCAGTTTGAAATCTAGCCCCTCGCGCAAACGCGCAGCTTTGATTTGCACCGGATCGCGTCTGGCCCCATATTGGAGGCATGACAGACAAGC
CCAAGAGACCCAAGGACGCGAACCAGTTGGCGAAGTTCATAACGGACTTGGCGACTGGTGAAGCATCTGAGGATGACGCCCCCGAAAAAGCCGAGGGTCA
GCGCAAGGGCGGCTTGAAGGGTGGCAGGGCGCGGGCTGACAGGCTGACGCCGGAAGAACGCAGCAAAATCGCCAAGAAGGCTGCGGCCGCCCGATGGCGG
AACGAAACCTGAATACCTGACATACACCCACCATACACCTCTTGACGTGATAGATTGAACGGTCTAGGAGTCCGGCAGCAGATTGGAGTTGCCGAAATGG
CCGTACTACTCACAACGGATCAGGTCGAGGAATGGAAGGCCGAGCGCGATAAGCTGGTAAAGCGGATCGCCTATCTTGATGAGCGCATCAAGGCCGCATC
CTTTTTCATGGCTGACGATGAAACAGAGAGCGAGCCGTCAGGTGATGATGGCCACCAGAGTTCGCCTGGGCCGTCTCTGGTGGACGCCATCGTTGATGTT
CTGCGGCGGTTTGGCCCCGAACCAATGACCAATCACGCCATCCGAAACCGGCTGACAGATGTTGGCTTTGACATTTCACAGATGAGCCAGAATTACTACT
ACACCGCCACGAAACGCTTGAGCGACAAGGGAACCATCGTGAAACACGATGACGGCACTTACTCGCTTCCTGAGAATGCGGCCCCGGAAGAATCTCTGTA
TAGCGACAGTTCTTCCGGGGCCAATATCGAGAGCCGGTTTAGCCAACCGACTGAACCCCCAGCGCAAGGCCGTGAAGCCGGCCCGGGAGGTGAGACATGA
GGAGGTAAGAACCTCATGTAAGTCGTCAGCCGCGCGGCGGGAGTTGGCCCTCCCAATGCGCGGCTGACGCATTTCTAGCATCCAAAACAGCAACTTGCAA
GGCGAGAATAGGTGATTCCCGTCAATGAGTTATGTCATCTGTGAATTTAAACTGACCGACATGCTTGACTATAGTATGGATAGCACCTACATTGTGAGTA
TGAACAAGTTGGATACCAAAACCCGCTCACTGATCATCCGCCTTTTGGTGGAAGGTAATTCAATTCGCGCCACATCGCGGATTGCTGACGTGAGCAAAAA
CACTGTCAACAAGCTGTTGATAGATGTGGGGAAAGTATGTCACTCGTACCACGATGGAAACGTGCGGGATGTAAAGGCGTCAGTCATCCAGTGCGACGAA
ATCTGGTCATTCACCTATGCCAAGCAAAAGAACGTTGCTGCTGCCAAGGCTGCGCCAGACGGCGCAGGCGACACGTGGACCTGGACGGCTCTGGATAGTG
ATAGCAAGCTGATCGTGTCCTACATGGTAGGTGGGCGCGACAGCGAATATGCTATTGGCTTCATGGACGATCTTTGCTCTCGGCTCGCCAACCGGGTCCA
ACTGACCACGGACGGTCACAAGGCATATCTGGAAGCCGTAGAAGGCGCATTCGGTGGCGACGTTGACTATGCCCAGCTCATCAAGCTGTACGGCGAACCC
ACGGGCCAGAAGGGGCACGAGCGCAAGTATTTTCCGTCAGAATGCACGGGCACCAAGAAACGTGCGGTGGAGGGTCGCCCCGACATGGCGAAGGTAAGCA
CGTCGCACGTTGAGCGGCAGAATCTTACCATGCGGATGCACATGCGCCGCTTTACGCGGCTGACTAACGGGTTCAGCAAGAAGGTTGAGAACCACGCCTA
TGCGGTCGCGCTGCACTTCATGTACTACAATTTCTGCCGCCAGCATCAAACGCTGTGCGTTTCGCCCGCAATGGCCGCCGGTCTGACGGATCGGCTTTGG
GATATTGCTGATCTGGTCGCGCTGGTGGACGCCGCAGAACCCGCGCCGAAGAAGCGCGGACCCTACAAAAAACGAGCGGTGAATTAATGCCAATCAAAGA
GCATCCAGCAATCGGCACGGTTTTGTTGTGTGACTTCACCTCGGGATTCTCGAAGCCCGAAATGCAAAAGCGGCGGCCGTCGTGGTGATCAGCCCAAAGA
TCACTGCTAGACCTTACCTGTGCACTGTCGTTGCCCTAAGCACGGACCCGCCAGTTCCCGTTCTGCCATATCACGCTCAAATCGACATTTTCCCAGAACT
TCCAAAGCCTTGGTCGAGCAAAGGCGTTTGGGTGAAGGGAGACATGGTCAACGCAGTCGGTTTTCATAGACTTGACCTGATCCGAACCGGAAAAGACCGG
GCGGGCAACCGAACTTACATCTATCAGCCCCTAAGTGAGGAAAATTTAAGAATAATTCGGGCGTGCGCTCTTTCAGCCTTGGGGTTGGCGCGCTTGACAA
AATCGCTGTGAGCGATTATTTGGGGTCCCGATAGCGGCTCTCACATGGTGATCCGCGACTAAGACCCCACCAGGGGCCGCCGTGCAACTTCGGTGATTGC
AAACTGAGCCGCACGGCGCAGATAGAAGACCCCGCTTCGGCGGGGTCTTTGCGTTGCAAGGGTTGTAACAGGCCAAGATTTCAAACTGACCCACTACC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
225 bp	74 aa	88	312	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTDKPKRPKDANQLAKFITDLATGEASEDDAPEKAEGQRKGGLKGGRARADRLTPEERSKIAKKAAAARWRNET

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
504 bp	167 aa	397	900	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAVLLTTDQVEEWKAERDKLVKRIAYLDERIKAASFFMADDETESEPSGDDGHQSSPGPSLVDAIVDVLRRFGPEPMTNHAIRNRLTDVGFDISQMSQNY
YYTATKRLSDKGTIVKHDDGTYSLPENAAPEESLYSDSSSGANIESRFSQPTEPPAQGREAGPGGET

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	1025	1987	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSYVICEFKLTDMLDYSMDSTYIVSMNKLDTKTRSLIIRLLVEGNSIRATSRIADVSKNTVNKLLIDVGKVCHSYHDGNVRDVKASVIQCDEIWSFTYAK
QKNVAAAKAAPDGAGDTWTWTALDSDSKLIVSYMVGGRDSEYAIGFMDDLCSRLANRVQLTTDGHKAYLEAVEGAFGGDVDYAQLIKLYGEPTGQKGHER
KYFPSECTGTKKRAVEGRPDMAKVSTSHVERQNLTMRMHMRRFTRLTNGFSKKVENHAYAVALHFMYYNFCRQHQTLCVSPAMAAGLTDRLWDIADLVAL
VDAAEPAPKKRGPYKKRAVN

Blast result :

Comments

ISOba3 is 38% aa similar to ISLysp1.
The transposase is the third ORF, others are passengers genes.

References

1] Giovannoni,S.J., Cho,J.-C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2005) Direct submission GenBank.