ISfinder

ISMul1

Family ISAs1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP000325	ND	Mycobacterium ulcerans	Mycobacterium ulcerans Agy99

DNA section

IS Length : 1366 bp

Ends

IR Length : 41

IRL : CAGGGCTCCGGCGTTGTTGATTAGCAGGCTTGTGAGCTGGGTTGATGATC
IRR : CAGGGCTCCGGCGTTGTTGATTAGCAGGCTTGTGAGCTGGGGAATTGTGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTATTCGGGTGCTGGGT		CTGGTCGAGGCTGGCTG	0
ACGCGGTCAA	CGCCCCCACC	CAGGCACTCCT	10
AGATGAGCCAGGCCTACAAG		GCTCGCTGCGCACG	0
TACCACGGCG	CCCAGCGCGC	GGAGTGTGAACAGGC	10

DNA sequence

CAGGGCTCCGGCGTTGTTGATTAGCAGGCTTGTGAGCTGGGTTGATGATCTTTGGTGCCGCGCTATTGGGGCCGCAGTGTAGGCACGGGCTATCTGGAGA
ACGATCGAGTTGGTTACTGTTCGTCGTTGGTCTGGAGTTGCCCGTGCCTACCGTTTCAGTATTGCCTGTGTCGTCGGTTCCAGAGGTGTTCGACCGCTGG
AGTCCTGGTGAACGCAGCGAGCTCATCGGATCGGAGTTTCTCGATGTGTTGGCCTCGGTGCCAGACCCGAGGGACCCACGCGGTCGGAGGTATTCGCTGA
TGGCTTTGTTGGCGATCGCGGTTCTGGCCACTGCCGCGGGGATGCGCGGCTATGCTGGTTTTGCCACATGGGCGGCCACCGCTTCCGATGATGTGTTGGC
CCAATTAGGGGTCCGGTTCCGGCGGCCCAGTGAGAAGACCTTCCGCGCTGTTTTGTCTCGGCTAGACCCCGCCGGCCTCAACGCCAGGATGGGCAGTTAC
TTCACTGCACACGTGGCCAGCAGCGACCCCAGTGGATTGGTGCCGATCGCGTTGGACGGCAAGATGCTGCGTGGTGCTTTACGCGCCAAAGCGACAGCCA
CGCATCTCGTGTCGGTGTTCGCCCACCGTGCCCGATTGGTGCCCGGTCAACTCGCTGTCGCCGAGAAAAGCAATGAAATTCCCTGCGTACGTGCCCTGCT
CACGCTGCTGCCGGATAACTTGCGGTGGCTGGTCACCGTGGATGCGATGCATACCCAGGTCGTCACCGCGAAGTTGATCTGCGCCACCTTGAAGTCGCAC
TACCTGATGATCGTCAAGTCCAACCAAGCCAAAATACTTGCCCGTATCACCGCGCTGCCCTGGGCCGAGGTGCCCGCAGCCGCTACCGACGACTCCCGCG
GCCACGGCCGTGTCAAGACCCGCACCCTGCAAATCATCACCGCTGCACGAGGAATCGGCTTCCCCTACGCAAAACAAATCATCCGGATCACTCGTGAACG
CTTGATCACCGCCACCGACCAGCGCAGCGTGGAGGTGGTCTATGCCATCTGCAGCCTGCCGTTCGAGCACGCCCGCCCTACCGCGATCATGACCTGGATG
CGTCAACACTGCGGAATCGAGAACAGCCTGCACTGGATACGCGACGTCACCTTCGACGAAGACCGTCACAGGGCACATACCGGAAACGGCGCACAGGTCC
TAGCAACGCTACGCAACACCGCGATCAATCTGCACCGCCTCAACGGCGCCGACAACATCGCCGAAGCCTGCCGGATCACCGCTTTGACCGCCAACCGCCG
CCTAGACCTCCTCAATCCACAATTCCCCAGCTCACAAGCCTGCTAATCAACAACGCCGGAGCCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1047 bp	327 aa	300	1346	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MALLAIAVLATAARMPYAGFATWAATASDDVLAQLGVRFRRPSEKTFRAVLSRLDPADLNARMGSYFTAHVASSDPSGLVPIELDGKNAAWCFTRQSDTT
HLVSVFAHRARLVLGQLAVAEKSNEIPCVRALLTLLPDNLRWLVTVDAMHTQVVTAKLICATLKSHYLMIVKSNQAKILARITALPWAEVPAAATDDSRG
HGRVKTRTLQIITAARGIGFPYAKQIIRITRERLITATDQRSVEVVYAICSLPFEHARPTAIMTWMRQHCGIENSLHWIRDVTFDEDRHRLHTGNGAQVL
ATRRNTAINLQPSTAPTTSPKPAGSPL

Blast result :

Comments

ISMul1 is 94% aa similar to IS2404.

References

1] Stinear,T.P., Seemann,T., Pidot,S.J.A., Frigui,W., Reysset,G., Garnier,T., Meurice,G., Simon,D., Bouchier,C., Ma,L., Tichit,M., Porter,J.L., Ryan,J.E., Johnson,P.D.R., Davies,J.K., Jenkin,G.A., Small,P.L.C., Jones,L.M., Tekaia,F., Laval,F., Daffe,M., Parkhill,J. and Cole,S.T. (2006) Direct submission GenBank.