ISMav4

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_002944 ND Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis K-10
DNA section
IS Length : 1089 bp

Ends


IR Length : 33/40

IRL : TGTACTGACCACGGACGTTGGTGACGGCGTGGTGAGGTGACATGACGAGA
IRR : TGTACTGACCTGAGAGGTTAGGTACGCGGCTGGCGGGTGGTTGGCCGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TAGTCCGTGCACTAGGGCTCAACGCG6

DNA sequence

TGTACTGACCACGGACGTTGGTGACGGCGTGGTGAGGTGACATGACGAGAACCTCCGAGTGAAGTGCGAGCTGCTTAGGAAACGCACTCACCTCGGAGGT
CCTCATGTCCCACCGTAATGCTCCATTGTCTGAAACCGGTCGGCTGCGGCTGGCCCGTTGCGTCGTTGATGAGGGTTGGTCGCTGCGGCGAGCAGCCGAA
CGCTTCCAGGTCTCGGTAACCACCGCAGAGCGCTGGGCCAGGCGCTACCGCGAGCTGGGCGAGGCCGGAATGGCCGACCGCAGCTCACGACCGCATCACA
GCCCCAACCGCACGCCCACGCGCACCGAGCGGCGCATCATCAAAGTCCGGGTGATCCGCCGGTGGGGACCAGCGCGCATCGGGTATCTGCTGGGGATACA
TCCCTCGACGGTCCATCGGGTATTGACCCGCTACGCGCTGGCCAAACTGCGCTGGCTGGACCGATCTACTGGCCGGATTATCCGGCGGATGGAACCGGCT
GGGTGCGGGGATCTAGTGCACGTCGATGTCAAGAAGCTCGGCAAAATCCCTGCCGGCGGCGGCTGGCGGATGCTGGGACGCGCGATTGGCGGCCACAACT
CCAACGCGGACAAGAGCAGCGGGGTGTTCAGCAAGCACCGCAACCCAATACGGGGATATCACTATCTCCACACCGCCATCGATGGATATTCCCGGCTGGC
CTACAGCGAGGTACTCGACGATGAGATCAAAGAAACCGCCGCCGAGTTCTGGACACGCGCTAATGCCTGGTTCGCCGAATGTGGGATCAGCGTGCGGAAA
GTGTTGACCGACAACGGGTCCTGCTACCGATCGCGCGTTTTTGCCCAGGCCCTGGGCGATATCGAACACCGCCGCACCCGACCCTACCGACCACAGACCA
ACGGCAAGGTCGAGAGATTTCACCGCACCTTGGCAGACGAATGGGCCTACGCCCGCCTCTACCGCAGTGACACTGAGCGCTGCGAAGAGTTCACCACCTG
GCTGCACACCTACAATCACCACCGCGGCCACACCGCACTTGGCGGCCAACCACCCGCCAGCCGCGTACCTAACCTCTCAGGTCAGTACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
990 bp329 aa1051094+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHRNAPLSETGRLRLARCVVDEGWSLRRAAERFQVSVTTAERWARRYRELGEAGMADRSSRPHHSPNRTPTRTERRIIKVRVIRRWGPARIGYLLGIHP
STVHRVLTRYALAKLRWLDRSTGRIIRRMEPAGCGDLVHVDVKKLGKIPAGGGWRMLGRAIGGHNSNADKSSGVFSKHRNPIRGYHYLHTAIDGYSRLAY
SEVLDDEIKETAAEFWTRANAWFAECGISVRKVLTDNGSCYRSRVFAQALGDIEHRRTRPYRPQTNGKVERFHRTLADEWAYARLYRSDTERCEEFTTWL
HTYNHHRGHTALGGQPPASRVPNLSGQYN

 

Blast result :
Comments
ISMav4 is 75 % aa similar to ISAni1 . The stop codon is in the DR.
References
1] Li,L., Bannantine,J.P., Zhang,Q., Amonsin,A., May,B.J., Alt,D., Banerji,N., Kanjilal,S. and Kapur,V. (2005) (er) Proc. Natl. Acad. Sci. U.S.A. In press