ISMxa2

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008095 ND Myxococcus xanthus
Myxococcus xanthus DK 1622
DNA section
IS Length : 1244 bp

Ends


IR Length : 28/44

IRL : TGGTCTGCCCCCCGATTTTCGGACCAGTTGAAGCGTGAGAGAGTCCTCGC
IRR : TGCTCTGCCCCCGTTCTTCGGACCACGCGTTACGAGAGTCCTGCGGCGCT

Insertion site


Left flankDirect repeatRight flankDR Length
TTCGATACAGCCGC0

DNA sequence

TGGTCTGCCCCCCGATTTTCGGACCAGTTGAAGCGTGAGAGAGTCCTCGCGGCCAGGAGGTAGGGCGGTCGTGAGAAAGAGCAGGTTTACCGAGGAGCAG
ATGGTCCGCATCCTGCGGGAGGCGGAGACGCCTGGGGCCAGCGTGGTGGAGGTGGCCCGGAAGCACGGCGTCGTCGAGCAGACGCTCTACCGGTGGCGGC
AGAAGTTCGGCGGCATGGAGGCAGGCGACGCGACGCGGCTCAGGGAGTTGGAGAAGGAGAACGCGCGTCTGAAGAAGCTGCTGGCCGAGAGGGACCTCGA
AATCGAGGTCATGAAGGAAATCTCCACAAAAAAATGGTGAGCGCGCCTGTCCGCCGTCGGCAGGTGCGGTACGCCATGGGGAAAGGCGTGTCGCAGCGCC
GGGCGTGCGCGCTACTCCAGGTGGCCGGCAGTAGCCTGGGCTACGCCAGCCGGAAAGAGGCAAAGGACGCGGCTCTGGTGGCGCAGTTGCGCGACATCGC
CCGGGCACGCCCCCGGTTCGGCTACCGTCGGGCCTGGGCCCTGCTGCGGCGCGAGGGGCCCGCGGTCAACGTGAAGCGAGTGCATCGGCTCTGGAGGAAG
GAGGGACTGGCGCTCTCTCGGAGACGGCCAAGGAAACGGCTTCGGCTGGGGCAGCAACGCCAACCCAAGCCGGAGGGCGTCAACTCGGTATGGGCCTGGG
ACTTCGTGCACGACAGGTGCGCAAATGGGCAGAAGCTCAAGTGCCTCACTGTCGTGGATGAACACAGCCGAGAGTGCCTGGCCATCGACGTGGCCGGGCG
CATCAGTGCCAGGCGCGTCATCGAGGTGCTCTCGCGCCTGGTAGCAGTGCACGGGCCGCCGAAGTACCTCAGAAGCGACAACGGCCCCGAGTTCATCGCC
AAGGCCCTGCGCAGGTGGCTGGAGGCCAATGGCATTCAGACGGCCTACATCGCTCCAGGCAAGCCCTGGCAGAACGGTACCAACGAAAGCTTCAACGGCC
GCTTCCGGGACGAGTGCCTGTCAGCGGAGTGGTTTTCCACCAGGAGGGAGGCCGTCGTCCTCATCGAGGCGTGGCGGCGCGACTACAACGAGAAGCGCCC
GCACAGCAGTTTGGGCTACAAGACACCTGCGGAGGTCGGAGCTCGCCGTGCCCATGCCGGCCCCGTCGCATCATCCCTCGAGCACGGCATCAGCAGCGCC
GCAGGACTCTCGTAACGCGTGGTCCGAAGAACGGGGGCAGAGCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa74340+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRKSRFTEEQMVRILREAETPGASVVEVARKHGVVEQTLYRWRQKFGGMEAGDATRLRELEKENARLKKLLAERDLEIEVMKEISTKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1152 bp383 aa641215+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GGREKEQVYRGADGPHPAGGGDAWGQRGGGGPEARRRRADALPVAAEVRRHGGRRRDAAQGVGEGERASEEAAGREGPRNRGHEGNLHKKMVSAPVRRRQ
VRYAMGKGVSQRRACALLQVAGSSLGYASRKEAKDAALVAQLRDIARARPRFGYRRAWALLRREGPAVNVKRVHRLWRKEGLALSRRRPRKRLRLGQQRQ
PKPEGVNSVWAWDFVHDRCANGQKLKCLTVVDEHSRECLAIDVAGRISARRVIEVLSRLVAVHGPPKYLRSDNGPEFIAKALRRWLEANGIQTAYIAPGK
PWQNGTNESFNGRFRDECLSAEWFSTRREAVVLIEAWRRDYNEKRPHSSLGYKTPAEVGARRAHAGPVASSLEHGISSAAGLS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1142 bp381 aa741215+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKSRFTEEQMVRILREAETPGASVVEVARKHGVVEQTLYRWRQKFGGMEAGDATRLRELEKENARLKKLLAERDLEIEVMKEISTKKMVSAPVRRRQVR
YAMGKGVSQRRACALLQVAGSSLGYASRKEAKDAALVAQLRDIARARPRFGYRRAWALLRREGPAVNVKRVHRLWRKEGLALSRRRPRKRLRLGQQRQPK
PEGVNSVWAWDFVHDRCANGQKLKCLTVVDEHSRECLAIDVAGRISARRVIEVLSRLVAVHGPPKYLRSDNGPEFIAKALRRWLEANGIQTAYIAPGKPW
QNGTNESFNGRFRDECLSAEWFSTRREAVVLIEAWRRDYNEKRPHSSLGYKTPAEVGARRAHAGPVASSLEHGISSAAGLS

 

Blast result :
Comments
ISMxa2 is 85% (ORFA) and 67% (ORFB) aa similar to ISBp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Goldman,B.S., Nierman,W.C., Kaiser,D., Slater,S.C., Durkin,A.S., Eisen,J.A., Ronning,C.M., Barbazuk,W.B., Blanchard,M., Field,C., Halling,C., Hinkle,G., Iartchuk,O., Kim,H.S., Mackenzie,C., Madupu,R., Miller,N., Shvartsbeyn,A., Sullivan,S.A., Vaudin,M., Wiegand,R. and Kaplan,H.B. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (41), 15200-15205.