ISOba1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAMO01000007 | ND | Oceanicola batsensis | Oceanicola batsensis HTCC2597 |
DNA section
IS Length : 1271 bp
Ends
IR Length : 34/45
IRL : TGACCTGCCACGGGATTTTTCCTCCACCGTCGATTAGAGTCCGGCCTATC
IRR : TGACCTGCCACTGAACTTTCATCCAGCCGCGACCGGAGCCCGGTTGGTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGACAGCATC | GTGGAACGCC | 0 |
DNA sequence
TGACCTGCCACGGGATTTTTCCTCCACCGTCGATTAGAGTCCGGCCTATCTTGAGGACGGACAATGAAGCGAACGAGATTCACGGACGAACAGATCATCG
GCATCCTGAGCGAGCACGAGGCTGGTGCGAAGTGCGCTGACCTGTGCCGCAAGCACGGCATGTCCGAAGGCACCTTCTACAACTGGAAGGCCAAGTTCGG
CGGCATGACGGTGTCAGAGGCCAAGCGGCTGAAGGCGCTGGAGGATGAGAACGCGAAGCTGAAGAAGCTGCTGGCCGAGCAAATGCTGGATCTGGCTGCG
ATGAAGGATCTGGTTTCAAAAAAGTGGTAGGGCCCGCCGTGAAGCGTGAAGCCGTCGCGTATCTTCGGGCCGAGCATGGCCTGTCGGAGCGGCGGGCCTG
CCATATCGTCGGCGCGGATCGAACGATGATACGCTACCGATCTCGGCGTGCCCCGGACACGGTTCTGCGCGGTCGGTTGCGGGACCTGGCCCACGAGCGG
CGGCGGTTCGGTTACCGACGCCTGTTCGTCCTGCTGCGCCGCGAGGGTGAGCCCTCGGGGATCAACCGGATCTATCGGCTCTACCGCGAGGAGGGGCTGA
CGGTGCGCAAGCGGAAGGCCCGGCGCAAGGCTGTCGGGACGCGAGCCCCGATCCTGGTCGAGGCGCGGCCCAATGCGCGCTGGTCGCTGGATTTCGTCCA
TGACCAATTCGCCAATGGCCAGCGCTTCCGGGTTCTCAACGTGGTCGACGACGTCACCCGTGAATGTCTCGCGGCAATACCGGACACCTCGATATCGGGG
CGCCGCGTGGCACGTGAACTGACGGCCCTGATCGAACGCCGTGGCAAACCAGGAATGATTGTCAGCGACAACGGCACGGAACTGACCTCCAACGCCATCC
TGCGGTGGTGTTCCGAGCACCGGATCGAATGGCACTACATCGCGCCAGGCAAGCCGATGCAGAACGGCTTCGTAGAGAGTTTCAACGGCCGGATGCGGGA
TGAACTGCTCAACGAGACCATGTTCCGGAACTTGGCCCACGCGCGGATCGTGATTGCCGCTTGGGCTGCCGACTACAATACAGAACGCCCGCATTCGGCC
TTGGACTACCAGACCCCGGCTGACTACGCACGGGCCCTGACCACCGCAATCGCCCGCCCCGCTGCGCGAGATGAAAGCTCCGCGCGTCGGGCGATTGCTA
AACCTGCGCCGATCGGCGTAAACACCAACCGGGCTCCGGTCGCGGCTGGATGAAAGTTCAGTGGCAGGTCA
GCATCCTGAGCGAGCACGAGGCTGGTGCGAAGTGCGCTGACCTGTGCCGCAAGCACGGCATGTCCGAAGGCACCTTCTACAACTGGAAGGCCAAGTTCGG
CGGCATGACGGTGTCAGAGGCCAAGCGGCTGAAGGCGCTGGAGGATGAGAACGCGAAGCTGAAGAAGCTGCTGGCCGAGCAAATGCTGGATCTGGCTGCG
ATGAAGGATCTGGTTTCAAAAAAGTGGTAGGGCCCGCCGTGAAGCGTGAAGCCGTCGCGTATCTTCGGGCCGAGCATGGCCTGTCGGAGCGGCGGGCCTG
CCATATCGTCGGCGCGGATCGAACGATGATACGCTACCGATCTCGGCGTGCCCCGGACACGGTTCTGCGCGGTCGGTTGCGGGACCTGGCCCACGAGCGG
CGGCGGTTCGGTTACCGACGCCTGTTCGTCCTGCTGCGCCGCGAGGGTGAGCCCTCGGGGATCAACCGGATCTATCGGCTCTACCGCGAGGAGGGGCTGA
CGGTGCGCAAGCGGAAGGCCCGGCGCAAGGCTGTCGGGACGCGAGCCCCGATCCTGGTCGAGGCGCGGCCCAATGCGCGCTGGTCGCTGGATTTCGTCCA
TGACCAATTCGCCAATGGCCAGCGCTTCCGGGTTCTCAACGTGGTCGACGACGTCACCCGTGAATGTCTCGCGGCAATACCGGACACCTCGATATCGGGG
CGCCGCGTGGCACGTGAACTGACGGCCCTGATCGAACGCCGTGGCAAACCAGGAATGATTGTCAGCGACAACGGCACGGAACTGACCTCCAACGCCATCC
TGCGGTGGTGTTCCGAGCACCGGATCGAATGGCACTACATCGCGCCAGGCAAGCCGATGCAGAACGGCTTCGTAGAGAGTTTCAACGGCCGGATGCGGGA
TGAACTGCTCAACGAGACCATGTTCCGGAACTTGGCCCACGCGCGGATCGTGATTGCCGCTTGGGCTGCCGACTACAATACAGAACGCCCGCATTCGGCC
TTGGACTACCAGACCCCGGCTGACTACGCACGGGCCCTGACCACCGCAATCGCCCGCCCCGCTGCGCGAGATGAAAGCTCCGCGCGTCGGGCGATTGCTA
AACCTGCGCCGATCGGCGTAAACACCAACCGGGCTCCGGTCGCGGCTGGATGAAAGTTCAGTGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 64 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MKRTRFTDEQIIGILSEHEAGAKCADLCRKHGMSEGTFYNWKAKFGGMTVSEAKRLKALEDENAKLKKLLAEQMLDLAAMKDLVSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1005 bp | 334 aa | 249 | 1253 | + | No |
Description : Second part of the transposase
ORF sequence :
EREAEEAAGRANAGSGCDEGSGFKKVVGPAVKREAVAYLRAEHGLSERRACHIVGADRTMIRYRSRRAPDTVLRGRLRDLAHERRRFGYRRLFVLLRREG
EPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVEARPNARWSLDFVHDQFANGQRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGK
PGMIVSDNGTELTSNAILRWCSEHRIEWHYIAPGKPMQNGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARALTTA
IARPAARDESSARRAIAKPAPIGVNTNRAPVAAG
EPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVEARPNARWSLDFVHDQFANGQRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGK
PGMIVSDNGTELTSNAILRWCSEHRIEWHYIAPGKPMQNGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARALTTA
IARPAARDESSARRAIAKPAPIGVNTNRAPVAAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 64 | 1253 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRTRFTDEQIIGILSEHEAGAKCADLCRKHGMSEGTFYNWKAKFGGMTVSEAKRLKALEDENAKLKKLLAEQMLDLAAMKDLVSKKVVGPAVKREAVAY
LRAEHGLSERRACHIVGADRTMIRYRSRRAPDTVLRGRLRDLAHERRRFGYRRLFVLLRREGEPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVE
ARPNARWSLDFVHDQFANGQRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGKPGMIVSDNGTELTSNAILRWCSEHRIEWHYIAPGKPMQ
NGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARALTTAIARPAARDESSARRAIAKPAPIGVNTNRAPVAAG
LRAEHGLSERRACHIVGADRTMIRYRSRRAPDTVLRGRLRDLAHERRRFGYRRLFVLLRREGEPSGINRIYRLYREEGLTVRKRKARRKAVGTRAPILVE
ARPNARWSLDFVHDQFANGQRFRVLNVVDDVTRECLAAIPDTSISGRRVARELTALIERRGKPGMIVSDNGTELTSNAILRWCSEHRIEWHYIAPGKPMQ
NGFVESFNGRMRDELLNETMFRNLAHARIVIAAWAADYNTERPHSALDYQTPADYARALTTAIARPAARDESSARRAIAKPAPIGVNTNRAPVAAG
Blast result :
Comments
Genome in progress.
ISOba1 is 86% (ORFA) and 81% (ORFB) aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISOba1 is 86% (ORFA) and 81% (ORFB) aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Giovannoni,S.J., Cho,J.-C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2005) Direct Submission GenBank.