ISMmy2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ887910 | Y | Mycoplasma mycoides | Mycoplasma yeatsii Mycoplasma mycoides subsp mycoides LC CIRAD-95010-C1 Mycoplasma mycoides Mycoplasma capricolum Mycoplasma cottewii |
DNA section
IS Length : 1374 bp
Ends
IR Length : 21/24
IRL : GAGAGTAGGACAAAATTATTAGACCCCCTAGGATAGACCCCCTAGGGGGT
IRR : GAAACCAGGACAAAATTATTAGACTAAAAAGTTATGTTTATTATACACAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | ATT | NNNNNNNNNN | 3 |
DNA sequence
GAGAGTAGGACAAAATTATTAGACCCCCTAGGATAGACCCCCTAGGGGGTCTATTTTTTTTGAAAAGGAGAACCTATGAAAAGAGGAAAACAGCTAAGTG
TAAATGAATGAATAGAACTGTTTAAACATTATAAAGATTATCTATCTCACAATATTACTAAAAAAGAATTTTATTATATTTATAGCAAAATTAGAAATTC
TGGTGATTCATTACCTTCACAAAAAGCTATGGAATATTTATCTAAAAAATATAGATATTATAATTTAGGTATGGAACTAAAAGAATCACAAACAGGAAGA
GCGCCTAAAAAAGGTAAGGGATCAGGTAGACCTAGAAAGAACAAAGAAGAATATGATAAACAAAGAAACGAAGTAATTAAGGAGATTGATCGTGAAGTTT
TAGAATGGTTAATAACAGATCTTTTTAAAGAAGAAATTTTAAAAAAGCATAAGGTTGATAATTTAGATGAATTAATAGAGAAAATTAAAGAGAAGTTTGG
AACAAATATTACTAATAAAAAAATAATGAAAATTCTTGGCCTACCTAAATCAACTTTTTACTACAAACTTAAAAAACAATCAAATGATAAGAATAAAGCG
TTTGTAAAACACGCAAACATAATTAAAGAAGCATTTGAAAAACAAAATGGAAGATACGGTAGAGAAAGGCTATCAGCATATATTTTCAAGACATATGGTA
TTAATATGAATCCCAGAACATTAGGAAGAGCAATGAATAAATTGGGTCTTTTTTGTCATGTGAGAACAATGTGCAAGAAAAAGCGTGAATCAAAAAATAC
TAACGTATATTATCCAAACATTGCTAATAGGGATTATAATGGGCTACAAAACGATATTTACGCAAGTGACGTTAGTTACATTCCAGCTCCAATTGACGTA
GAAGGAAGACACGTTTATTTATCTATCTTAATTCATCACAGAACAAAGAAAGTTGTTAGCTATAATTTATCTATAAATAACGATACTAATTTGGTTATGT
CTCACATTGTAAAAACAAAATTTCCTAAAGACTTTATTATTCATACAGATCATGGTGCTGCATATTCATCAATAGAATATCTTGACTACATAAAACAACA
AGGTGGAATAGTGTCAATGTCAAGAATAGGCAATTCACTAGACAATAGAGAGGCTGAGTATTTCTTTTCAATTTTAAAATCAGAGATGTTTATTAACTTT
GAAAAGAAGGTAAAAGAAATTACCTTTGGCGAACTAAAAGAATACATTAAAAATTTTATTGAATGATATAACAGTGAAAGAATTTTAAGAAAATTCAAAT
GAAAAACACCTCAAGAATTATGAGGTGTGTATAATAAACATAACTTTTTAGTCTAATAATTTTGTCCTGGTTTC
TAAATGAATGAATAGAACTGTTTAAACATTATAAAGATTATCTATCTCACAATATTACTAAAAAAGAATTTTATTATATTTATAGCAAAATTAGAAATTC
TGGTGATTCATTACCTTCACAAAAAGCTATGGAATATTTATCTAAAAAATATAGATATTATAATTTAGGTATGGAACTAAAAGAATCACAAACAGGAAGA
GCGCCTAAAAAAGGTAAGGGATCAGGTAGACCTAGAAAGAACAAAGAAGAATATGATAAACAAAGAAACGAAGTAATTAAGGAGATTGATCGTGAAGTTT
TAGAATGGTTAATAACAGATCTTTTTAAAGAAGAAATTTTAAAAAAGCATAAGGTTGATAATTTAGATGAATTAATAGAGAAAATTAAAGAGAAGTTTGG
AACAAATATTACTAATAAAAAAATAATGAAAATTCTTGGCCTACCTAAATCAACTTTTTACTACAAACTTAAAAAACAATCAAATGATAAGAATAAAGCG
TTTGTAAAACACGCAAACATAATTAAAGAAGCATTTGAAAAACAAAATGGAAGATACGGTAGAGAAAGGCTATCAGCATATATTTTCAAGACATATGGTA
TTAATATGAATCCCAGAACATTAGGAAGAGCAATGAATAAATTGGGTCTTTTTTGTCATGTGAGAACAATGTGCAAGAAAAAGCGTGAATCAAAAAATAC
TAACGTATATTATCCAAACATTGCTAATAGGGATTATAATGGGCTACAAAACGATATTTACGCAAGTGACGTTAGTTACATTCCAGCTCCAATTGACGTA
GAAGGAAGACACGTTTATTTATCTATCTTAATTCATCACAGAACAAAGAAAGTTGTTAGCTATAATTTATCTATAAATAACGATACTAATTTGGTTATGT
CTCACATTGTAAAAACAAAATTTCCTAAAGACTTTATTATTCATACAGATCATGGTGCTGCATATTCATCAATAGAATATCTTGACTACATAAAACAACA
AGGTGGAATAGTGTCAATGTCAAGAATAGGCAATTCACTAGACAATAGAGAGGCTGAGTATTTCTTTTCAATTTTAAAATCAGAGATGTTTATTAACTTT
GAAAAGAAGGTAAAAGAAATTACCTTTGGCGAACTAAAAGAATACATTAAAAATTTTATTGAATGATATAACAGTGAAAGAATTTTAAGAAAATTCAAAT
GAAAAACACCTCAAGAATTATGAGGTGTGTATAATAAACATAACTTTTTAGTCTAATAATTTTGTCCTGGTTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1278 bp | 426 aa | 76 | 1353 | + | No |
Chemistry : DDE
ORF sequence :
MKRGKQLSVNEWIELFKHYKDYLSHNITKKEFYYIYSKIRNSGDSLPSQKAMEYLSKKYRYYNLGMELKESQTGRAPKKGKGSGRPRKNKEEYDKQRNEV
IKEIDREVLEWLITDLFKEEILKKHKVDNLDELIEKIKEKFGTNITNKKIMKILGLPKSTFYYKLKKQSNDKNKAFVKHANIIKEAFEKQNGRYGRERLS
AYIFKTYGINMNPRTLGRAMNKLGLFCHVRTMCKKKRESKNTNVYYPNIANRDYNGLQNDIYASDVSYIPAPIDVEGRHVYLSILIHHRTKKVVSYNLSI
NNDTNLVMSHIVKTKFPKDFIIHTDHGAAYSSIEYLDYIKQQGGIVSMSRIGNSLDNREAEYFFSILKSEMFINFEKKVKEITFGELKEYIKNFIEWYNS
ERILRKFKWKTPQELWGVYNKHNFLV
IKEIDREVLEWLITDLFKEEILKKHKVDNLDELIEKIKEKFGTNITNKKIMKILGLPKSTFYYKLKKQSNDKNKAFVKHANIIKEAFEKQNGRYGRERLS
AYIFKTYGINMNPRTLGRAMNKLGLFCHVRTMCKKKRESKNTNVYYPNIANRDYNGLQNDIYASDVSYIPAPIDVEGRHVYLSILIHHRTKKVVSYNLSI
NNDTNLVMSHIVKTKFPKDFIIHTDHGAAYSSIEYLDYIKQQGGIVSMSRIGNSLDNREAEYFFSILKSEMFINFEKKVKEITFGELKEYIKNFIEWYNS
ERILRKFKWKTPQELWGVYNKHNFLV
Blast result :
Comments
ISMmy2 is 60% aa similar to IS1138. The ends of this IS are not the expected ends for one IS3 family member.
The original host contains 6 copies of this IS.
The other copies are 1373 bp long.
The direct repeats for the other copies are: GGT; TAA
The original host contains 6 copies of this IS.
The other copies are 1373 bp long.
The direct repeats for the other copies are: GGT; TAA
References
1] Thiaucourt. F. (2006) Direct submission.