ISMma10
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1171 bp
Ends
IR Length : 23/30
IRL : TGTCTCAACCATTTAATTATTAGTCATCATTATAAACGTTGAATCCTTTT
IRR : TGTCATGACCATTTAATTATTTCACATAATACTTTGACATTTTCTCATCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCAGTTTCGGGGA | CTAG | TCCAGAATGTAAAATCT | 4 |
DNA sequence
TGTCTCAACCATTTAATTATTAGTCATCATTATAAACGTTGAATCCTTTTATACCTTCATGCTACAGATTCAACTTGCAGATAATGAGGTAAATTTCCTT
CAGGATTTTGTGAGAAAAGGACGTAAAAGCGCAAGAGAACTAACTCGAGCCCGTATTCTCCTTCTATCAAACCAGCAAACAGAAATCACTGAAATCGTGA
AAATATTAGGTATTTCCAGGAGTACCACCTTGAATATACGGAAAAGATACCTTGATGAAGGTATCCCCAATGCTCTTTTTGACAAATCAAGATCTGGTCA
ACCTATAAAATACACTGAAAAACATGTTGCAGAAGTAATCGCTTTAGCCTGCAGCAGTTCCCCTGATGGAAGCAAGAGATGGTCGCTATCCCTGCTTACT
GAAGAACTTAGGAAAAAAGAAGGATTTGAAACTATAGGTAAAGAGAGTGTTCGGCTTATTTTGAAAAAAGCAAAACTAAACCTTGGTTAAGAAGGATGTG
GTGTATTCAGACTATCGATACTGAATATAGAGACAGGATGTATGACATTCTTAGTCTGTATGAAGAGGATTATGATCCTAAAAAACCTCTTATCTGTCTT
GACGAGAAGCCTAAGCAACTGCTCAGGGATAAAAGAATGAGCATTCCTATGAAGCTTGGAAGCTCAGAAAAATATGATTATGAGTATGTCAGGAACGGAA
CAGCAAACATATTCATGGCGGTAGAGTTCAAAGCAGGAAAAAGGGTAACTCAGGTAACCAAAAGAAGAACAATGAAGGATTTTGCACAATTCATGAAGAT
TCTTGTCACAGAAAAATATTCTGAAGCAGAAGTCATCAGACTGGTTACGGATAATCTCAATATCCATAAAGAGAAGTCATTCTACGAAGCATTCTCAGAA
GAGGAAGCAAAGAAGATTCTGGACAAGATAGAGTTCCATTACACGCCAAAACATGCAAGCTGGCTCAATGCTGCTGAAATCGAGATCAATGTCATGGATA
TCGAATGTACAGGAAGAAGGATCGGGAACATCGAGACGCTTAAAAATGAAGTGGATTCATGGACAAAGAGAAGGAATGAACACAAAAGGAAAATTGAATG
GAAGTTTACAAGGAAGAATGCAGATGAGAAAATGTCAAAGTATTATGTGAAATAATTAAATGGTCATGACA
CAGGATTTTGTGAGAAAAGGACGTAAAAGCGCAAGAGAACTAACTCGAGCCCGTATTCTCCTTCTATCAAACCAGCAAACAGAAATCACTGAAATCGTGA
AAATATTAGGTATTTCCAGGAGTACCACCTTGAATATACGGAAAAGATACCTTGATGAAGGTATCCCCAATGCTCTTTTTGACAAATCAAGATCTGGTCA
ACCTATAAAATACACTGAAAAACATGTTGCAGAAGTAATCGCTTTAGCCTGCAGCAGTTCCCCTGATGGAAGCAAGAGATGGTCGCTATCCCTGCTTACT
GAAGAACTTAGGAAAAAAGAAGGATTTGAAACTATAGGTAAAGAGAGTGTTCGGCTTATTTTGAAAAAAGCAAAACTAAACCTTGGTTAAGAAGGATGTG
GTGTATTCAGACTATCGATACTGAATATAGAGACAGGATGTATGACATTCTTAGTCTGTATGAAGAGGATTATGATCCTAAAAAACCTCTTATCTGTCTT
GACGAGAAGCCTAAGCAACTGCTCAGGGATAAAAGAATGAGCATTCCTATGAAGCTTGGAAGCTCAGAAAAATATGATTATGAGTATGTCAGGAACGGAA
CAGCAAACATATTCATGGCGGTAGAGTTCAAAGCAGGAAAAAGGGTAACTCAGGTAACCAAAAGAAGAACAATGAAGGATTTTGCACAATTCATGAAGAT
TCTTGTCACAGAAAAATATTCTGAAGCAGAAGTCATCAGACTGGTTACGGATAATCTCAATATCCATAAAGAGAAGTCATTCTACGAAGCATTCTCAGAA
GAGGAAGCAAAGAAGATTCTGGACAAGATAGAGTTCCATTACACGCCAAAACATGCAAGCTGGCTCAATGCTGCTGAAATCGAGATCAATGTCATGGATA
TCGAATGTACAGGAAGAAGGATCGGGAACATCGAGACGCTTAAAAATGAAGTGGATTCATGGACAAAGAGAAGGAATGAACACAAAAGGAAAATTGAATG
GAAGTTTACAAGGAAGAATGCAGATGAGAAAATGTCAAAGTATTATGTGAAATAATTAAATGGTCATGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 432 bp | 143 aa | 59 | 490 | + | No |
Description : First part of the transposase
ORF sequence :
MLQIQLADNEVNFLQDFVRKGRKSARELTRARILLLSNQQTEITEIVKILGISRSTTLNIRKRYLDEGIPNALFDKSRSGQPIKYTEKHVAEVIALACSS
SPDGSKRWSLSLLTEELRKKEGFETIGKESVRLILKKAKLNLG
SPDGSKRWSLSLLTEELRKKEGFETIGKESVRLILKKAKLNLG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 660 bp | 219 aa | 496 | 1155 | + | No |
Description : Second part of the transposase
ORF sequence :
MWCIQTIDTEYRDRMYDILSLYEEDYDPKKPLICLDEKPKQLLRDKRMSIPMKLGSSEKYDYEYVRNGTANIFMAVEFKAGKRVTQVTKRRTMKDFAQFM
KILVTEKYSEAEVIRLVTDNLNIHKEKSFYEAFSEEEAKKILDKIEFHYTPKHASWLNAAEIEINVMDIECTGRRIGNIETLKNEVDSWTKRRNEHKRKI
EWKFTRKNADEKMSKYYVK
KILVTEKYSEAEVIRLVTDNLNIHKEKSFYEAFSEEEAKKILDKIEFHYTPKHASWLNAAEIEINVMDIECTGRRIGNIETLKNEVDSWTKRRNEHKRKI
EWKFTRKNADEKMSKYYVK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1097 bp | 0 aa | 59 | 1155 | + | Yes |
Chemistry : DDE
ORF sequence :
Blast result :
Comments
ISMma10 is 89% aa similar to ISMac17.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baeumer,S., Jacobi,C., Brueggemann,H., Lienard,T., Christmann,A., Boemecke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.-P., Gunsalus,R.P., Fritz,H.-J. and Gottschalk,G. (2002)J. Mol. Microbiol. Biotechnol.