ISMma9
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1200 bp
Ends
IR Length : 15/22
IRL : TGTCGTGTCACCGATGGAAAGTCTTAAGCGTTATATTGAATGATCTTCAT
IRR : TGTCGCGTCAATCCTCAATAGTCGGATAAAGACGTGACAATTTTATCCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTTAAGAGA | CTAA | GCTATGCAGG | 4 |
| TTACAACCGA | GTAG | CCTGTTTTTC | 4 |
| TGACGCGACA | CTAG | CTTCATTCTT | 4 |
| CTTAAAATTA | CTAG | TAACGAATCT | 4 |
| TACTTCAAGG | CTAG | TACAATTTCA | 4 |
| AGGAAAAACA | CTAG | TGAGTTACAG | 4 |
DNA sequence
TGTCGTGTCACCGATGGAAAGTCTTAAGCGTTATATTGAATGATCTTCATAAGTATCATTCATGATTAAATATACTGTGACACTTACCGAAGATGAACGT
AGATCTCTTTGTGAACTTGCTTCAAAGGGAAAACATAACTCTCAACAAATTCTCAATGCTCTGATTTTGCTTAATTGCGATAAAAGTGAATTGAATGTTA
GCCATTCAACCAATGAAGAAATCTCACGTGTCCTGAATATTAGTATGAAAAAAATTGACCGTGTTAAGAAGAGATTTGTTGAAGAAGGTCTTGAAGTTGC
CCTTAACGGGAAAGAAAGCGAGCGCATTTATAATAAAAAAGTTGATGGTGATCTTGAAGCCCATTTAGTCGCCCTTAGTTGCAGTCAACCCCCTGAAGGT
TTTGCAAGATGGTCGTTAAGATTATTAGCCGATAAGGCTGTAGAACTTGGTTATTTCGAGGAAATTTCTCACGAAACAGTACGCCGTACTTTAAAAAAAC
GAAATCAAACCCTGGCAAAGGAAACAATGGGTAATTCCTCCAGAACAAAACAGTAGCTTTGTTGCGAATATGGAAATGGTTCTGGACGTTTATAAACGTC
CATTTGATCCACGAAATCCTGTTGTGTGTATGGACGAATCCCCAAAACAATTGATTGCAGAAACCCGGATCCCTATCCCCTGCTCAACAGGGAAGCCAGA
AAAGTACGATTATGAATACAAGCGAAATGGAATGTGCAATATATTCCTTGCCTGTGAACCGTTGACAGGGAAAAGAATGGTAAAGATCACTGAAAGAAAA
ACAAAGAGAGATTGGGCTTATTTTCTCGAAGAAATAGAAGTTCAACATGAAAATGCAGATAAAATTACGTTAGTAATGGACAATCTAAACACGCACATAC
CTGGATCTCTCTACGAAACATTTCCACCACCAAAAGCAAAGGCGCTATGGGACAGATTTGAGTTCGTCTACACGCCAAAACATGGAAGCTGGTTGAACAT
GGCAGAGATTGAACTGAATGTACTTACAGGTCAATGTTTAAAAAGGAGAATGGATAACATCGAGTTTGTCAGAAAAGAAGTTTTGGCATGGCAAAATTAC
AGAAACAACAAAAATTCAAAGGTTAAATGGCAATTTACAACAGATGATGCAAGGATAAAATTGTCACGTCTTTATCCGACTATTGAGGATTGACGCGACA
AGATCTCTTTGTGAACTTGCTTCAAAGGGAAAACATAACTCTCAACAAATTCTCAATGCTCTGATTTTGCTTAATTGCGATAAAAGTGAATTGAATGTTA
GCCATTCAACCAATGAAGAAATCTCACGTGTCCTGAATATTAGTATGAAAAAAATTGACCGTGTTAAGAAGAGATTTGTTGAAGAAGGTCTTGAAGTTGC
CCTTAACGGGAAAGAAAGCGAGCGCATTTATAATAAAAAAGTTGATGGTGATCTTGAAGCCCATTTAGTCGCCCTTAGTTGCAGTCAACCCCCTGAAGGT
TTTGCAAGATGGTCGTTAAGATTATTAGCCGATAAGGCTGTAGAACTTGGTTATTTCGAGGAAATTTCTCACGAAACAGTACGCCGTACTTTAAAAAAAC
GAAATCAAACCCTGGCAAAGGAAACAATGGGTAATTCCTCCAGAACAAAACAGTAGCTTTGTTGCGAATATGGAAATGGTTCTGGACGTTTATAAACGTC
CATTTGATCCACGAAATCCTGTTGTGTGTATGGACGAATCCCCAAAACAATTGATTGCAGAAACCCGGATCCCTATCCCCTGCTCAACAGGGAAGCCAGA
AAAGTACGATTATGAATACAAGCGAAATGGAATGTGCAATATATTCCTTGCCTGTGAACCGTTGACAGGGAAAAGAATGGTAAAGATCACTGAAAGAAAA
ACAAAGAGAGATTGGGCTTATTTTCTCGAAGAAATAGAAGTTCAACATGAAAATGCAGATAAAATTACGTTAGTAATGGACAATCTAAACACGCACATAC
CTGGATCTCTCTACGAAACATTTCCACCACCAAAAGCAAAGGCGCTATGGGACAGATTTGAGTTCGTCTACACGCCAAAACATGGAAGCTGGTTGAACAT
GGCAGAGATTGAACTGAATGTACTTACAGGTCAATGTTTAAAAAGGAGAATGGATAACATCGAGTTTGTCAGAAAAGAAGTTTTGGCATGGCAAAATTAC
AGAAACAACAAAAATTCAAAGGTTAAATGGCAATTTACAACAGATGATGCAAGGATAAAATTGTCACGTCTTTATCCGACTATTGAGGATTGACGCGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 495 bp | 164 aa | 62 | 556 | + | No |
Description : First part of the transposase
ORF sequence :
MIKYTVTLTEDERRSLCELASKGKHNSQQILNALILLNCDKSELNVSHSTNEEISRVLNISMKKIDRVKKRFVEEGLEVALNGKESERIYNKKVDGDLEA
HLVALSCSQPPEGFARWSLRLLADKAVELGYFEEISHETVRRTLKKRNQTLAKETMGNSSRTKQ
HLVALSCSQPPEGFARWSLRLLADKAVELGYFEEISHETVRRTLKKRNQTLAKETMGNSSRTKQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 624 bp | 207 aa | 570 | 1193 | + | No |
Description : Second part of the transposase
ORF sequence :
MEMVLDVYKRPFDPRNPVVCMDESPKQLIAETRIPIPCSTGKPEKYDYEYKRNGMCNIFLACEPLTGKRMVKITERKTKRDWAYFLEEIEVQHENADKIT
LVMDNLNTHIPGSLYETFPPPKAKALWDRFEFVYTPKHGSWLNMAEIELNVLTGQCLKRRMDNIEFVRKEVLAWQNYRNNKNSKVKWQFTTDDARIKLSR
LYPTIED
LVMDNLNTHIPGSLYETFPPPKAKALWDRFEFVYTPKHGSWLNMAEIELNVLTGQCLKRRMDNIEFVRKEVLAWQNYRNNKNSKVKWQFTTDDARIKLSR
LYPTIED
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1132 bp | 377 aa | 62 | 1193 | + | Yes |
Chemistry : DDE
ORF sequence :
MIKYTVTLTEDERRSLCELASKGKHNSQQILNALILLNCDKSELNVSHSTNEEISRVLNISMKKIDRVKKRFVEEGLEVALNGKESERIYNKKVDGDLEA
HLVALSCSQPPEGFARWSLRLLADKAVELGYFEEISHETVRRTLKKNEIKPWQRKQWVIPPEQNSSFVANMEMVLDVYKRPFDPRNPVVCMDESPKQLIA
ETRIPIPCSTGKPEKYDYEYKRNGMCNIFLACEPLTGKRMVKITERKTKRDWAYFLEEIEVQHENADKITLVMDNLNTHIPGSLYETFPPPKAKALWDRF
EFVYTPKHGSWLNMAEIELNVLTGQCLKRRMDNIEFVRKEVLAWQNYRNNKNSKVKWQFTTDDARIKLSRLYPTIED
HLVALSCSQPPEGFARWSLRLLADKAVELGYFEEISHETVRRTLKKNEIKPWQRKQWVIPPEQNSSFVANMEMVLDVYKRPFDPRNPVVCMDESPKQLIA
ETRIPIPCSTGKPEKYDYEYKRNGMCNIFLACEPLTGKRMVKITERKTKRDWAYFLEEIEVQHENADKITLVMDNLNTHIPGSLYETFPPPKAKALWDRF
EFVYTPKHGSWLNMAEIELNVLTGQCLKRRMDNIEFVRKEVLAWQNYRNNKNSKVKWQFTTDDARIKLSRLYPTIED
Blast result :
Comments
ISMma9 is 62% aa similar to ISMth1.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baeumer,S., Jacobi,C., Brueggemann,H., Lienard,T., Christmann,A., Boemecke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.-P., Gunsalus,R.P., Fritz,H.-J. and Gottschalk,G. (2002)J. Mol. Microbiol. Biotechnol.