ISMma16
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1268 bp
Ends
IR Length : 21/25
IRL : GGCAGTGTACCGAAAGTTCTGTAAATTATGATTGACTCTGTATCCTTTTC
IRR : GGCAGTGTGCCTAATTTTCTGTAAAATTTGCTCTATACCCCTTAGTCTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTTCATATTTT | GTTCAGAT | AATGTCTACATATT | 8 |
DNA sequence
GGCAGTGTACCGAAAGTTCTGTAAATTATGATTGACTCTGTATCCTTTTCCTTTCACCACAAAAAAACAGGATGCAGAGTCAATGGTAGATTTATTCTCA
CTCATAAAAGATTATCTTGTCGATCAGGAAGATGGAATTCGCCATTTAATTACGTGGTTTTTAAATCTCGTAATGGAGGAGGAAGCCCTCCTCCAGGTTG
GTGCACAACGTTATGAGAGGACTGATTCTCGTAAAGCCAGTAGAAATGGCTACAAACCCCGAACCCTCCTTACCAAGTATGGAGAACTTGAATTATTAAA
ACCTCAATTCCGTGAGTTTCCCTTTGAAACTCAGGTATTTGAGAAATATTCCCGAGTTGAACAGGCCATCTTATCCGCTGTAGCTGAATCTTACCTGCAA
GGCGTTTCCACCCGAAGGGTGGATAAGGTCATGACTGCTTTGGGAGTTGAGGGAATCTCAGCCTCTTCAGTTTCCAGAATTACAAAAGAGCTGGATGAGA
AAGTTTCAGAATTTTTGTCAAAGCCAATAGAACACGAAATTCCTTATTTGTTTATTGATGCTACTTATCTCAAAGTAAGAGATGGACTTCATTACGAAAA
TAAAGCTCTCTTTATAGTTTCTGGAGTCAGGGATGATGGTTTTCGCGAGATTCTTGGAGCAAGATTGGCAGACAGCGAAGACTCTCTGTTCTGGCAAGAT
CTATTCGAAGACCTGAAAGAAAGAGGGTTAAGAGGTGTCAAGTTAATTGTTTCTGATGGTCATAAAGGAATACAAAAAGCAGTTAGAGAATCCTTCATCG
GATCAAGCTGGCAGATGTGCCACGTTCATCTGATAAGGCAAGCTCTAAAAAAGGTTCAAAAAAAGAAGCAAAAAGAAGTAGCAGATAAGATAAAAGAAGC
ATTGGTAGACCGTCAAAAATACAATGATTTGATAAGGGAACTGGATAAGATGGAATATAAAAGTGCAGCTGATACTCTTGAGAATTTTCAGTATGATGTA
ATGAATTACATGCAGTTTCCACAGAGTCATTGGAGAAAAATAAGGACGACAAATATGATGGAGAGAACTAACAAGGAGATAAAAAGAAGAACCAAAGTTG
TAGGAGCATTCCCTAACCAGGAATCAGTATTGAGACTGGTAGTCTCAATACTCATTGATATAAATGAAGACTGGATTACTGGAAACAGGTATATAGTAAT
GGAGCAGTAATCAAAAAAGAAGACTAAGGGGTATAGAGCAAATTTTACAGAAAATTAGGCACACTGCC
CTCATAAAAGATTATCTTGTCGATCAGGAAGATGGAATTCGCCATTTAATTACGTGGTTTTTAAATCTCGTAATGGAGGAGGAAGCCCTCCTCCAGGTTG
GTGCACAACGTTATGAGAGGACTGATTCTCGTAAAGCCAGTAGAAATGGCTACAAACCCCGAACCCTCCTTACCAAGTATGGAGAACTTGAATTATTAAA
ACCTCAATTCCGTGAGTTTCCCTTTGAAACTCAGGTATTTGAGAAATATTCCCGAGTTGAACAGGCCATCTTATCCGCTGTAGCTGAATCTTACCTGCAA
GGCGTTTCCACCCGAAGGGTGGATAAGGTCATGACTGCTTTGGGAGTTGAGGGAATCTCAGCCTCTTCAGTTTCCAGAATTACAAAAGAGCTGGATGAGA
AAGTTTCAGAATTTTTGTCAAAGCCAATAGAACACGAAATTCCTTATTTGTTTATTGATGCTACTTATCTCAAAGTAAGAGATGGACTTCATTACGAAAA
TAAAGCTCTCTTTATAGTTTCTGGAGTCAGGGATGATGGTTTTCGCGAGATTCTTGGAGCAAGATTGGCAGACAGCGAAGACTCTCTGTTCTGGCAAGAT
CTATTCGAAGACCTGAAAGAAAGAGGGTTAAGAGGTGTCAAGTTAATTGTTTCTGATGGTCATAAAGGAATACAAAAAGCAGTTAGAGAATCCTTCATCG
GATCAAGCTGGCAGATGTGCCACGTTCATCTGATAAGGCAAGCTCTAAAAAAGGTTCAAAAAAAGAAGCAAAAAGAAGTAGCAGATAAGATAAAAGAAGC
ATTGGTAGACCGTCAAAAATACAATGATTTGATAAGGGAACTGGATAAGATGGAATATAAAAGTGCAGCTGATACTCTTGAGAATTTTCAGTATGATGTA
ATGAATTACATGCAGTTTCCACAGAGTCATTGGAGAAAAATAAGGACGACAAATATGATGGAGAGAACTAACAAGGAGATAAAAAGAAGAACCAAAGTTG
TAGGAGCATTCCCTAACCAGGAATCAGTATTGAGACTGGTAGTCTCAATACTCATTGATATAAATGAAGACTGGATTACTGGAAACAGGTATATAGTAAT
GGAGCAGTAATCAAAAAAGAAGACTAAGGGGTATAGAGCAAATTTTACAGAAAATTAGGCACACTGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1179 bp | 392 aa | 32 | 1210 | + | No |
Chemistry : DDE
ORF sequence :
MTLYPFPFTTKKQDAESMVDLFSLIKDYLVDQEDGIRHLITWFLNLVMEEEALLQVGAQRYERTDSRKASRNGYKPRTLLTKYGELELLKPQFREFPFET
QVFEKYSRVEQAILSAVAESYLQGVSTRRVDKVMTALGVEGISASSVSRITKELDEKVSEFLSKPIEHEIPYLFIDATYLKVRDGLHYENKALFIVSGVR
DDGFREILGARLADSEDSLFWQDLFEDLKERGLRGVKLIVSDGHKGIQKAVRESFIGSSWQMCHVHLIRQALKKVQKKKQKEVADKIKEALVDRQKYNDL
IRELDKMEYKSAADTLENFQYDVMNYMQFPQSHWRKIRTTNMMERTNKEIKRRTKVVGAFPNQESVLRLVVSILIDINEDWITGNRYIVMEQ
QVFEKYSRVEQAILSAVAESYLQGVSTRRVDKVMTALGVEGISASSVSRITKELDEKVSEFLSKPIEHEIPYLFIDATYLKVRDGLHYENKALFIVSGVR
DDGFREILGARLADSEDSLFWQDLFEDLKERGLRGVKLIVSDGHKGIQKAVRESFIGSSWQMCHVHLIRQALKKVQKKKQKEVADKIKEALVDRQKYNDL
IRELDKMEYKSAADTLENFQYDVMNYMQFPQSHWRKIRTTNMMERTNKEIKRRTKVVGAFPNQESVLRLVVSILIDINEDWITGNRYIVMEQ
Blast result :
Comments
ISMma16 is 60% aa similar to ISDha1.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baeumer,S., Jacobi,C., Brueggemann,H., Lienard,T., Christmann,A., Boemecke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.-P., Gunsalus,R.P., Fritz,H.-J. and Gottschalk,G. (2002)J. Mol. Microbiol. Biotechnol.