ISMbu1

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ZP_0014730 ND Methanococcoides burtonii
Methanococcoides burtonii
DNA section
IS Length : 1141 bp

Ends


IR Length : 17/22

IRL : GAGAATTCCGATAAACCTCGGAAACAGACGCAATATTTATAATATATTAC
IRR : GAGAATTGCGATTAACTGCTGATTTTTTTCAAAGCAAATTTTTCCAGCTA

Insertion site


Left flankDirect repeatRight flankDR Length
TAATAAATAGAATATACTTAAGCAAAGCTC4

DNA sequence

GAGAATTCCGATAAACCTCGGAAACAGACGCAATATTTATAATATATTACAATAATTTTGTATTATGTCCTTAACAAACTTTGCTTTTAAAGAAGAGTAC
AAACGTCTTGAAAATCTCGGTGACAAGCTCTCTGAAATTGAATCTCTCATCGATTGGAAACCATTTCGTCCAATTATAGCAGAGATGTATATCAATAAAA
CAGAGTTCGGTGGCAGACCAAACGTTGATGAAATCGTCATGCTCAAAATGTTAGTATTGCAACAATGGCATGGACTATCTGACCCTGAACTTGAAAGACA
AGCTACTGACAGAATTTCCTTTAGGAAATTCTTGGGCTTTCCTGCAAAAATTCCAGATCATACTACTGTTTGGGCATTTAGAGAACGAATTGCTCAATCA
GGAAAAGAAGATGAAATCTGGAATGAAATGCAAAGACAACTTGATAAGAAAGGTTTGAGGATCAAACAAGGTATGATTCAGGATGCAACATTTATACATG
CTGATCCAGGACATGCAAATCTTGATACTCCTCGTGGAAATGAAGCAAAGACCAGAAGATGTAAGGACGGTACATGGACAAAAAAGGCATCTAAGTCACA
TTTTGGATATAAACTACATACCATTGAAGATACCGAATATGATCTGATAAGGAGATATAGGACAACTACTGCCTCAGTTCATGATAGTCAGGTGGATCTT
TCTGAAGAAGGCGAAGTTGTTTACAGAGATAGAGGTTACTTTGGTGCAATTTCAAAAGGATATGATGCAACTATGCAAAGGGGCGTGCGAGGTCATCCTA
TTGGTATTAGGGATAAGATGAGAAACAAAAGAATAAGCAGGAAAAGAGCAAAGGGAGAAAGACCTTATGCTGTTATCAAAAATGTGTTTACGTCAGGATT
TGTAAGAGTAACAACGTTGGCAAGAGTAAATGTCAAAATGGCGATTACAGCATTCAGCTATAATCTCTATCAATTGAGGACAATAAGAAGAAAATCATTA
GGATGAATAGCGGTAGCTATTCAAAAAAGTTGGAAAGTATATAAATAGATACTAGCAAACTGCTGGAAATAGAGAGAAAAGCTCAAAATTTTAGCTGGAA
AAATTTGCTTTGAAAAAAATCAGCAGTTAATCGCAATTCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
939 bp313 aa691007+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSLTNFAFKEEYKRLENLGDKLSEIESLIDWKPFRPIIAEMYINKTEFGGRPNVDEIVMLKMLVLQQWHGLSDPELERQATDRISFRKFLGFPAKIPDHT
TVWAFRERISQAGKEDEIWNEMQRQLNKKGLKIKQGMIQDATFIHADPGHANLDTPRGNEAKTRRCKDGTWTKKASKSHFGYKLHTIEDTEYDLIRRYRT
TTASVHDSQVDLSEEGEVVYRDRGYFGAISKGYDATMQRGVRGHPIGIRDKMRNKRISRKRAKGERPYAVIKNVFTSGFVRVTTLARVNVKMAITAFSYN
LYQLRTIRRKSLG

 

Blast result :
Comments
ISMbu1 is 52% aa similar to IS1194.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T.,
Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission to Genbank