Transposase expression and activity
While many of the classical mechanisms of
controlling gene expression, such as the production of transcriptional
repressors (IS1: (Machida & Machida, 1989`) (Escoubas, et
al., 1991); IS2: (Hu, et al., 1994) or translational inhibitors
(anti-sense RNA in the case of IS10; see (Kleckner, et al., 1996) are known to operate in Tpase
expression, several other mechanisms have also been uncovered.
References :
- Escoubas JM, Prere MF, Fayet O, Salvignol I, Galas D,
Zerbib D & Chandler M (1991) Translational control of transposition
activity of the bacterial insertion sequence IS1. Embo J 10: 705-712.
- Hu ST, Hwang JH, Lee LC, Lee CH, Li PL & Hsieh YC
(1994) Functional analysis of the 14 kDa protein of insertion sequence 2. J Mol.Biol. 236: 503-513.
- Kleckner N, Chalmers RM, Kwon D, Sakai J & Bolland
S (1996) Tn10 and IS10 Transposition and Chromosome Rearrangements: Mechanisms
and Regulation in Vivo and In Vitro. Transposable
elements,(Saedler H & Gierl A, ed.eds.), p.pp. 49-82. Springer,
Heidelberg.
- Machida
C & Machida Y (1989) Regulation of IS1 transposition by the insA gene
product. J Mol.Biol. 208: 567-574.